Review



antisense mo hem1  (Gene Tools Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Gene Tools Inc antisense mo hem1
    Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng <t>antisense</t> MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), <t>Hem1</t> MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).
    Antisense Mo Hem1, supplied by Gene Tools Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antisense mo hem1/product/Gene Tools Inc
    Average 90 stars, based on 1 article reviews
    antisense mo hem1 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish"

    Article Title: Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish

    Journal: Biology Open

    doi: 10.1242/bio.035576

    Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng antisense MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), Hem1 MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).
    Figure Legend Snippet: Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng antisense MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), Hem1 MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).

    Techniques Used: Injection, Staining, Expressing, Quantitation Assay, Fluorescence, Generated, In Vivo



    Similar Products

    antisense mo hem1  (Gene Tools Inc)
    90
    Gene Tools Inc antisense mo hem1
    Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng <t>antisense</t> MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), <t>Hem1</t> MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).
    Antisense Mo Hem1, supplied by Gene Tools Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antisense mo hem1/product/Gene Tools Inc
    Average 90 stars, based on 1 article reviews
    antisense mo hem1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng antisense MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), Hem1 MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).

    Journal: Biology Open

    Article Title: Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish

    doi: 10.1242/bio.035576

    Figure Lengend Snippet: Morpholino targeting of Hemogen inhibits erythropoiesis in embryonic zebrafish. Embryos were injected with 2 to 4 ng antisense MO targeted to the first 25 coding nucleotides of Hemogen . (A-B) O -dianisidine staining of erythrocytes was decreased in morphants (MO) relative to wild-type embryos (WT) or embryos rescued with 500 pg synthetic Hemgn mRNA (zHem) at 24 hpf. (ANOVA, Tukey post hoc test, P <0.001). (C-E) Live wild-type (C), Hem1 MO-injected (D) and Hem1 mm mismatch MO-injected (E) Tg(Lcr:EGFP) cz3325Tg embryos at 20 hpf. Morphants showed decreased EGFP expression in the ICM compared to the wild-type and mismatch MO controls. (F-H) Live wild-type (F), Hem1 MO-injected (G) and Hem1 mm MO-injected (H) embryos at 72 hpf. Morphant embryos have fewer EGFP+ cells in circulation compared to the two controls. The dorsal aortas of embryos (insets above F-H) were magnified 20× to permit quantitation of EGFP+ erythrocytes. Background red (D,G) and green (E,H) fluorescence was generated by the fluorescent labels on the MOs. (I) In vivo flow quantitation of EGFP+ erythrocyte concentrations between 3 and 6 dpf in Hem1-injected ( n =9,7,7,7), Hem1mm-injected ( n =13,14,11,11) and uninjected ( n =5,10,10,9) embryos. Data shown as means±s.e.m. (* P ≤0.05, ** P ≤0.001, ANOVA, Tukey-Kramer post hoc test). Arrowheads show notochord kinking. Scale bars: 500 µm (A-F); 100 µm (inset).

    Article Snippet: The antisense MO Hem1 (5′-TCTCTTTCTCCAACGGGTCTTCCAT-3′), which targets the first 25 base pairs of the zebrafish Hemogen open reading frame, was designed according to the manufacturer's instructions (Gene Tools LLC, Philomath, USA).

    Techniques: Injection, Staining, Expressing, Quantitation Assay, Fluorescence, Generated, In Vivo